pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)
(Plasmid #114212)

• Purpose: Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex
• Depositing Lab: Xue Han
• Publication: Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049.

Backbone

• Vector backbone: pHR (lentivirus)
• Total vector size (bp): 10089
• Modifications to backbone: 8 miR-128 target sites and 8 miR-221 target sites included in 3' UTR
• Vector type: Mammalian Expression, Mouse Targeting, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescent Protein
• Alt name: EGFP
• Species: M. musculus (mouse)
• Insert Size (bp): 717
• Mutation: None
• Promoter: human synapsin (hSyn)
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry