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Addgene

lenti EF1a-FLAG-dCas9-VPR
(Plasmid #114195)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-VPR
  • Species
    Synthetic
  • Insert Size (bp)
    5865
  • Promoter EF1a
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene #63798 into lentivirus backbone and addition of N-term FLAG tag and EF1a promoter . Please visit https://www.biorxiv.org/content/early/2018/07/17/371500 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti EF1a-FLAG-dCas9-VPR was a gift from Jeremy Day (Addgene plasmid # 114195 ; http://n2t.net/addgene:114195 ; RRID:Addgene_114195)

  • For your References section:

    A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation. Savell KE, Bach SV, Zipperly ME, Revanna JS, Goska NA, Tuscher JJ, Duke CG, Sultan FA, Burke JN, Williams D, Ianov L, Day JJ. eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-0495-18. doi: 10.1523/ENEURO.0495-18.2019. eCollection 2019 Jan-Feb. 10.1523/ENEURO.0495-18.2019 PubMed 30863790
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