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PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA FRT TO
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Backbone manufacturerThermo Fisher Scientific
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAmyloid-Precursor-Protein
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Alt nameAPP
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SpeciesH. sapiens (human)
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Insert Size (bp)2088
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MutationPopular mouse model, overexpress 2 mutations: Swedish + Indiana :Indiana= V717F (GTG---TTC); Swedish= KM670/671NL (AAG ATG---AAT CTG)
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Entrez GeneAPP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pc DNA FRT TO- APPSwed/Ind was a gift from Aleksandra Radenovic (Addgene plasmid # 114194 ; http://n2t.net/addgene:114194 ; RRID:Addgene_114194)