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Paxillin_PSCFP2_ mEOS2
(Plasmid #114192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmEOS2-Paxillin
  • Modifications to backbone
    PSCFP2 has been PCR amplified then cloned into PXN-meos2 plasmid using EcoRI and BamHI RE.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Paxillin
  • Alt name
    PXN
  • Species
    G. gallus (chicken)
  • Entrez Gene
    PXN (a.k.a. TGFB1I1)
  • Promoter SV40
  • Tags / Fusion Proteins
    • PS-CFP2 (C terminal on insert)
    • mEos2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Paxillin_PSCFP2_ mEOS2 was a gift from Aleksandra Radenovic (Addgene plasmid # 114192 ; http://n2t.net/addgene:114192 ; RRID:Addgene_114192)
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