pcDNA-Flag1-3HABeta2-mEos2
(Plasmid #114187)

• Purpose: studying clustering of Beta2 receptors by superresolution microscopy (PALM)
• Depositing Lab: Aleksandra Radenovic
• Publication: Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi: 10.1038/nmeth.1627.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5424
• Modifications to backbone: mEOS2 has been fused at the C term of 3HA beta2 into pcDNA3.1 vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Adrenergic receptor beta 2
• Alt name: Beta2
• Alt name: ADRB2
• Species: H. sapiens (human)
• Insert Size (bp): 1326
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • 3HA (N terminal on insert)
  • mEOS2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry