M3-PSCFP2
(Plasmid #114185)

• Purpose: studying clustering of M3 receptors by superresolution microscopy (PALM)
• Depositing Lab: Aleksandra Radenovic
• Publication: Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-80. doi: 10.1074/jbc.M111.329912. Epub 2012 Mar 22.

Backbone

• Vector backbone: pPS-CFP2-N
• Backbone manufacturer: Evrogen
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: pcDNA3.1-M3 receptor (wt) was purchased from cDNA.org. They amplified M3 by PCR from human whole brain cDNA (Clontech).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Muscarin acetylcholine receptor 3
• Alt name: M3
• Alt name: CHRM3
• Species: H. sapiens (human)
• Insert Size (bp): 1790
• Entrez Gene: CHRM3 (a.k.a. EGBRS, HM3, PBS, m3AChR)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xHA (N terminal on insert)
  • PS-CFP2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry