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Addgene

ORAI1-YFP T184M-L273D-L276D
(Plasmid #114184)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114184 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFP-N1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    Generated by site-directed mutagenesis on plasmid Orai1 YFP (Anjana Rao, Addgene #19756).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORAI1-YFP T184M-L273D-L276D
  • Species
    H. sapiens (human)
  • Mutation
    T184M/L273D/L276D
  • Entrez Gene
    ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ORAI1-YFP T184M-L273D-L276D was a gift from Nicolas Demaurex (Addgene plasmid # 114184 ; http://n2t.net/addgene:114184 ; RRID:Addgene_114184)

  • For your References section:

    ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain. Bulla M, Gyimesi G, Kim JH, Bhardwaj R, Hediger MA, Frieden M, Demaurex N. J Physiol. 2018 Nov 1. doi: 10.1113/JP277079. 10.1113/JP277079 PubMed 30382595
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