ORAI1-YFP L273D-L276D
(Plasmid
#114182)
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PurposeORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneEGFP-N1
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Backbone manufacturerClontech
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Modifications to backboneGenerated by site-directed mutagenesis on plasmid Orai1 YFP (Anjana Rao, Addgene #19756).
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORAI1-YFP L273D-L276D
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SpeciesH. sapiens (human)
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MutationL273D/L276D
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Entrez GeneORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ORAI1-YFP L273D-L276D was a gift from Nicolas Demaurex (Addgene plasmid # 114182 ; http://n2t.net/addgene:114182 ; RRID:Addgene_114182) -
For your References section:
ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain. Bulla M, Gyimesi G, Kim JH, Bhardwaj R, Hediger MA, Frieden M, Demaurex N. J Physiol. 2018 Nov 1. doi: 10.1113/JP277079. 10.1113/JP277079 PubMed 30382595
