ORAI1-YFP T184M
(Plasmid #114181)

• Purpose: ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).
• Depositing Lab: Nicolas Demaurex
• Publication: Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP277079.

Backbone

• Vector backbone: EGFP-N1
• Backbone manufacturer: Clontech
• Modifications to backbone: Generated by site-directed mutagenesis on plasmid Orai1 YFP (Anjana Rao, Addgene #19756).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ORAI1-YFP T184M
• Species: H. sapiens (human)
• Mutation: T184M
• Entrez Gene: ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry