p2K7-bsd-UBI-tagRFP-KDEL
(Plasmid #114179)

• Purpose: Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER
• Depositing Lab: Nicolas Demaurex
• Publication: Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6.

Backbone

• Vector backbone: p2K7
• Backbone manufacturer: David Suter (PMID:16293575)
• Backbone size w/o insert (bp): 8776
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tagRFP-KDEL
• Alt name: ER-RFP
• Species: Synthetic
• Insert Size (bp): 831
• Promoter: Ubiquitin
• Tags / Fusion Proteins:
  • signal peptide (N terminal on insert)
  • KDEL retention signal (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry