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p2K7bsdUBI-mCherry-STIM1
(Plasmid #114178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2K7
  • Backbone manufacturer
    David Suter (PMID:16293575)
  • Backbone size w/o insert (bp) 8776
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STIM1
  • Alt name
    GOK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2774
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Promoter Ubiquitin
  • Tag / Fusion Protein
    • mCherry (inserted after signal peptide) (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2K7bsdUBI-mCherry-STIM1 was a gift from Nicolas Demaurex (Addgene plasmid # 114178 ; http://n2t.net/addgene:114178 ; RRID:Addgene_114178)

  • For your References section:

    STIM1 promotes migration, phagosomal maturation and antigen cross-presentation in dendritic cells. Nunes-Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, Guido D, Bermont F, Bassoy EY, Page N, Merkler D, Hugues S, Martinvalet D, Manoury B, Demaurex N. Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6. 10.1038/s41467-017-01600-6 [pii] PubMed 29176619
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