Skip to main content
Addgene

pENTR1a-mCherry-STIM1
(Plasmid #114176)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114176 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pENTR1a
  • Backbone manufacturer
    Invitrogen/Thermo
  • Backbone size w/o insert (bp) 2717
  • Vector type
    Gateway entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STIM1
  • Alt name
    GOK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2774
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Tag / Fusion Protein
    • mCherry (inserted after signal peptide) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1a-mCherry-STIM1 was a gift from Nicolas Demaurex (Addgene plasmid # 114176 ; http://n2t.net/addgene:114176 ; RRID:Addgene_114176)

  • For your References section:

    STIM1 promotes migration, phagosomal maturation and antigen cross-presentation in dendritic cells. Nunes-Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, Guido D, Bermont F, Bassoy EY, Page N, Merkler D, Hugues S, Martinvalet D, Manoury B, Demaurex N. Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6. 10.1038/s41467-017-01600-6 [pii] PubMed 29176619
Related items:
Commonly requested with: