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Addgene

pET29b-clbI S178A
(Plasmid #114159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Total vector size (bp) 8304
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    clbI
  • Species
    E. coli
  • Mutation
    Mutated S178 to Ala
  • GenBank ID
    CAJ76291
  • Tags / Fusion Proteins
    • 6xHis-Thrombin site (N terminal on insert)
    • 6xHis (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-clbI S178A was a gift from Emily Balskus (Addgene plasmid # 114159 ; http://n2t.net/addgene:114159 ; RRID:Addgene_114159)

  • For your References section:

    Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring. Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus EP. Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 10.1038/nchembio.2448. Epub 2017 Aug 7. 10.1038/nchembio.2448 PubMed 28805802
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