Skip to main content
Addgene

pET29b-clbH-C-A2-PCP S1524A
(Plasmid #114158)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET29b
  • Total vector size (bp) 8711
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    clbH
  • Species
    E. coli
  • Mutation
    Only contains the C-A2-PCP domains of ClbH with S1524A mutation
  • GenBank ID
    CAJ76292
  • Tag / Fusion Protein
    • S-Tag, Thrombin site (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-clbH-C-A2-PCP S1524A was a gift from Emily Balskus (Addgene plasmid # 114158 ; http://n2t.net/addgene:114158 ; RRID:Addgene_114158)

  • For your References section:

    Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring. Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus EP. Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 10.1038/nchembio.2448. Epub 2017 Aug 7. 10.1038/nchembio.2448 PubMed 28805802
Related items: