pET28a-clbH-C-A2-PCP
(Plasmid
#114157)
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PurposeExpresses ClbH-C-A2-PCP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Total vector size (bp) 8711
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameclbH
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SpeciesE. coli
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MutationOnly contains the C-A2-PCP domains of ClbH
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GenBank IDCAJ76292
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Tag
/ Fusion Protein
- 6xHis-thrombin-T7 tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-clbH-C-A2-PCP was a gift from Emily Balskus (Addgene plasmid # 114157 ; http://n2t.net/addgene:114157 ; RRID:Addgene_114157) -
For your References section:
Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring. Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus EP. Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 10.1038/nchembio.2448. Epub 2017 Aug 7. 10.1038/nchembio.2448 PubMed 28805802