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pET28a-clbK
(Plasmid #114152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Total vector size (bp) 11788
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    clbK
  • Species
    E. coli
  • GenBank ID
    CAJ76289
  • Tag / Fusion Protein
    • 6xhis-Thrombin (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-clbK was a gift from Emily Balskus (Addgene plasmid # 114152 ; http://n2t.net/addgene:114152 ; RRID:Addgene_114152)

  • For your References section:

    Characterization of Polyketide Synthase Machinery from the pks Island Facilitates Isolation of a Candidate Precolibactin. Zha L, Wilson MR, Brotherton CA, Balskus EP. ACS Chem Biol. 2016 May 20;11(5):1287-95. doi: 10.1021/acschembio.6b00014. Epub 2016 Mar 1. 10.1021/acschembio.6b00014 PubMed 26890481
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