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pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
(Plasmid #114144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown
  • Species
    M. musculus (mouse)
  • Mutation
    G2A mutation and silent mutation to resist shRNA knockdown
  • Entrez Gene
    Prkaca (a.k.a. PKCD, Pkaca)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_shRNA resistant nmPKA-Cα-mEGFP was a gift from Haining Zhong (Addgene plasmid # 114144 ; http://n2t.net/addgene:114144 ; RRID:Addgene_114144)

  • For your References section:

    Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates. Tillo SE, Xiong WH, Takahashi M, Miao S, Andrade AL, Fortin DA, Yang G, Qin M, Smoody BF, Stork PJS, Zhong H. Cell Rep. 2017 Apr 18;19(3):617-629. doi: 10.1016/j.celrep.2017.03.070. 10.1016/j.celrep.2017.03.070 PubMed 28423323
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