Skip to main content
Addgene

pEFU6-EGFP_shPKA-Cα
(Plasmid #114142)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114142 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEFU6-EGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA expression for knock down mouse PKA-Cα
  • gRNA/shRNA sequence
    aacttgaattctccttcaa
  • Species
    M. musculus (mouse)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFU6-EGFP_shPKA-Cα was a gift from Haining Zhong (Addgene plasmid # 114142 ; http://n2t.net/addgene:114142 ; RRID:Addgene_114142)
  • For your References section:

    Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates. Tillo SE, Xiong WH, Takahashi M, Miao S, Andrade AL, Fortin DA, Yang G, Qin M, Smoody BF, Stork PJS, Zhong H. Cell Rep. 2017 Apr 18;19(3):617-629. doi: 10.1016/j.celrep.2017.03.070. 10.1016/j.celrep.2017.03.070 PubMed 28423323