pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
(Plasmid
#114111)
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Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114111 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 6235
- Total vector size (bp) 8446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameGaussia luciferase, slow burn
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Alt namesbGLuc
Gene/Insert 2
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Gene/Insert nameGuillardia theta anion channelrhodopsin-2, human codon optimized
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Alt namehGtARC2
Gene/Insert 3
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Gene/Insert nameEnhanced Yellow Fluorescent Protein
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Alt nameEYFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP was a gift from Ute Hochgeschwender (Addgene plasmid # 114111 ; http://n2t.net/addgene:114111 ; RRID:Addgene_114111) -
For your References section:
Defining parameters of specificity for bioluminescent optogenetic activation of neurons using in vitro multi electrode arrays (MEA). Prakash M, Medendorp WE, Hochgeschwender U. J Neurosci Res. 2018 Aug 28. doi: 10.1002/jnr.24313. 10.1002/jnr.24313 PubMed 30152529