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Addgene

pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
(Plasmid #114111)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114111 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 6235
  • Total vector size (bp) 8446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Gaussia luciferase, slow burn
  • Alt name
    sbGLuc

Gene/Insert 2

  • Gene/Insert name
    Guillardia theta anion channelrhodopsin-2, human codon optimized
  • Alt name
    hGtARC2

Gene/Insert 3

  • Gene/Insert name
    Enhanced Yellow Fluorescent Protein
  • Alt name
    EYFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP was a gift from Ute Hochgeschwender (Addgene plasmid # 114111 ; http://n2t.net/addgene:114111 ; RRID:Addgene_114111)
  • For your References section:

    Defining parameters of specificity for bioluminescent optogenetic activation of neurons using in vitro multi electrode arrays (MEA). Prakash M, Medendorp WE, Hochgeschwender U. J Neurosci Res. 2018 Aug 28. doi: 10.1002/jnr.24313. 10.1002/jnr.24313 PubMed 30152529