pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP
(Plasmid #114106)

• Purpose: fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics
• Depositing Lab: Ute Hochgeschwender
• Publication: Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002/jnr.24424.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4610
• Total vector size (bp): 6881
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Gaussia luciferase, slow burn
• Alt name: sbGLuc

Gene/Insert 2

• Gene/Insert name: Channelrhodopsin 2(CS/DA), stable step function opsin
• Alt name: ChR2(CS/DA)

Gene/Insert 3

• Gene/Insert name: Enhanced Yellow Fluorescent Protein
• Alt name: EYFP

Resource Information

• Supplemental Documents:
  • pAAV hSyn sbGLuc ChR2(CS-DA) EYFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry