pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)
(Plasmid #114103)

• Purpose: fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
• Depositing Lab: Ute Hochgeschwender
• Publication: Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi: 10.3389/fneur.2021.792643

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4552
• Total vector size (bp): 6888
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Gaussia luciferase, slow burn
• Alt name: sbGLuc

Gene/Insert 2

• Gene/Insert name: Volvox Channelrhodopsin 1
• Alt name: VChR1

Gene/Insert 3

• Gene/Insert name: Enhanced Yellow Fluorescent Protein
• Alt name: EYFP

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry