pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)
(Plasmid
#114103)
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Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4552
- Total vector size (bp) 6888
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameGaussia luciferase, slow burn
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Alt namesbGLuc
Gene/Insert 2
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Gene/Insert nameVolvox Channelrhodopsin 1
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Alt nameVChR1
Gene/Insert 3
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Gene/Insert nameEnhanced Yellow Fluorescent Protein
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Alt nameEYFP
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP) was a gift from Ute Hochgeschwender (Addgene plasmid # 114103 ; http://n2t.net/addgene:114103 ; RRID:Addgene_114103) -
For your References section:
Restoring Function After Severe Spinal Cord Injury Through BioLuminescent-OptoGenetics. Petersen ED, Sharkey ED, Pal A, Shafau LO, Zenchak-Petersen J, Pena AJ, Aggarwal A, Prakash M, Hochgeschwender U. Front Neurol. 2022 Jan 20;12:792643. doi: 10.3389/fneur.2021.792643 10.3389/fneur.2021.792643 PubMed 35126293