pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077) Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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> Addgene NGS Result ggtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatata tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggag tatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacg tcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggca gtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctcccc atctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatggggg cggggggggggggggsgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagag gtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcg gccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctcc gccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacg gcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtga aagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtg tgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggc tttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctg cgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtc gggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctcc gtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggc ggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcga ggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtccc aaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtg cggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccct ctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggct tctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagct cctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattctgcagtcgacggtaccgc gggcccgggatccaccggtcgccaccatgacacagttcgagggctttaccaacctgtatcaggtgagcaa gacactgcggtttgagctgatcccacagggcaagaccctgaagcacatccaggagcagggcttcatcgag gaggacaaggcccgcaatgatcactacaaggagctgaagcccatcatcgatcggatctacaagacctatg ccgaccagtgcctgcagctggtgcagctggattgggagaacctgagcgccgccatcgactcctatagaaa ggagaaaaccgaggagacaaggaacgccctgatcgaggagcaggccacatatcgcaatgccatccacgac tacttcatcggccggacagacaacctgaccgatgccatcaataagagacacgccgagatctacaagggcc tgttcaaggccgagctgtttaatggcaaggtgctgaagcagctgggcaccgtgaccacaaccgagcacga gaacgccctgctgcggagcttcgacaagtttacaacctacttctccggcttttatagaaacaggaagaac gtgttcagcgccgaggatatcagcacagccatcccacaccgcatcgtgcaggacaacttccccaagttta aggagaattgtcacatcttcacacgcctgatcaccgccgtgcccagcctgcgggagcactttgagaacgt gaagaaggccatcggcatcttcgtgagcacctccatcgaggaggtgttttccttccctttttataaccag ctgctgacacagacccagatcgacctgtataaccagctgctgggaggaatctctcgggaggcaggcaccg agaagatcaagggcctgaacgaggtgctgaatctggccatccagaagaatgatgagacagcccacatcat cgcctccctgccacacagattcatccccctgtttaagcagatcctgtccgataggaacaccctgtctttc atcctggaggagtttaagagcgacgaggaagtgatccagtccttctgcaagtacaagacactgctgagaa acgagaacgtgctggagacagccgaggccctgtttaacgagctgaacagcatcgacctgacacacatctt catcagccacaagaagctggagacaatcagcagcgccctgtgcgaccactgggatacactgaggaatgcc ctgtatgagcggagaatctccgagctgacaggcaagatcaccaagtctgccaaggagaaggtgcagcgca gcctgaagcacgaggatatcaacctgcaggagatcatctctgccgcaggcaaggagctgagcgaggcctt caagcagaaaaccagcgagatcctgtcccacgcacacgccgccctggatcagccactgcctacaaccctg aagaagcaggaggagaaggagatcctgaagtctcagctggacagcctgctgggcctgtaccacctgctgg actggtttgccgtggatgagtccaacgaggtggaccccgagttctctgcccggctgaccggcatcaagct ggagatggagccttctctgagcttctacaacaaggccagaaattatgccaccaagaagccctactccgtg gagaagttcaagctgaactttcagatgcctacactggccagaggctgggacgtgaataaggagaagaaca atggcgccatcctgtttgtgaagaacggcctgtactatctgggcatcatgccaaagcagaagggcaggta taaggccctgagcttcgagcccacagagaaaaccagcgagggctttgataagatgtactatgactacttc cctgatgccgccaagatgatcccaagatgcagcacccagctgaaggccgtgacagcccactttcagaccc acacaacccccatcctgctgtccaacaatttcatcgagcctctggagatcacaaaggagatctacgacct gaacaatcctgagaaggagccaaagaagtttcagacagcctacgccaagaaaaccggcgaccagaagggc tacagagaggccctgtgcaagtggatcgacttcacaagggattttctgtccaagtataccaagacaacct ctatcgatctgtctagcctgcggccatcctctcagtataaggacctgggcgagtactatgccgagctgaa tcccctgctgtaccacatcagcttccagagaatcgccgagaaggagatcatggatgccgtggagacaggc aagctgtacctgttccagatctataacaaggactttgccaagggccaccacggcaagcctaatctgcaca cactgtattggaccggcctgttttctccagagaacctggccaagacaagcatcaagctgaatggccaggc cgagctgttctaccgccctaagtccaggatgaagaggatggcacaccggctgggagagaagatgctgaac aagaagctgaaggatcagaaaaccccaatccccgacaccctgtaccaggagctgtacgactatgtgaatc acagactgtcccacgacctgtctgatgaggccagggccctgctgcccaacgtgatcaccaaggaggtgtc tcacgagatcatcaaggataggcgctttaccagcgacaagttctttttccacgtgcctatcacactgaac tatcaggccgccaattccccatctaagttcaaccagagggtgaatgcctacctgaaggagcaccccgaga cacctatcatcggcatcgatcggggcgagagaaacctgatctatatcacagtgatcgactccaccggcaa gatcctggagcagcggagcctgaacaccatccagcagtttgattaccagaagaagctggacaacagggag aaggagagggtggcagcaaggcaggcctggtctgtggtgggcacaatcaaggatctgaagcagggctatc tgagccaggtcatccacgagatcgtggacctgatgatccactaccaggccgtggtggtgctggagaacct gaatttcggctttaagagcaagaggaccggcatcgccgagaaggccgtgtaccagcagttcgagaagatg ctgatcgataagctgaattgcctggtgctgaaggactatccagcagagaaagtgggaggcgtgctgaacc cataccagctgacagaccagttcacctcctttgccaagatgggcacccagtctggcttcctgttttacgt gcctgccccatatacatctaagatcgatcccctgaccggcttcgtggaccccttcgtgtggaaaaccatc aagaatcacgagagccgcaagcacttcctggagggcttcgactttctgcactacgacgtgaaaaccggcg acttcatcctgcactttaagatgaacagaaatctgtccttccagaggggcctgcccggctttatgcctgc atgggatatcgtgttcgagaagaacgagacacagtttgacgccaagggcacccctttcatcgccggcaag agaatcgtgccagtgatcgagaatcacagattcaccggcagataccgggacctgtatcctgccaacgagc tgatcgccctgctggaggagaagggcatcgtgttcagggatggctccaacatcctgccaaagctgctgga gaatgacgattctcacgccatcgacaccatggtggccctgatccgcagcgtgctgcagatgcggaactcc aatgccgccacaggcgaggactatatcaacagccccgtgcgcgatctgaatggcgtgtgcttcgactccc ggtttcagaacccagagtggcccatggacgccgatgccaatggcgcctaccacatcgccctgaagggcca gctgctgctgaatcacctgaaggagagcaaggatctgaagctgcagaacggcatctccaatcaggactgg ctggcctacatccaggagctgcgcaacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaaga aaaagggatcctacccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcataccc atatgatgtccccgactatgcctaaagcggccgcactcctcaggtgcaggctgcctatcagaaggtggtg gctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatgggg acatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtg ttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgag tatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagag gtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggtta gattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgtttta ctagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccc tcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctca caattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaact cacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatc tcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgc ccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgag ctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgc agcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcat tctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcgg ccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgc tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcag gggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgtt gctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtgg cgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttc cgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgtt cagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgc cactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaa gtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacc ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgttt gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctga cgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctag atccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagtt accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgact ccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcga gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtg gtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgcc agttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatg gcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcgg ttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggc agcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaacc aagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccg cgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggat cttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttact ttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacac ggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcat gagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaa gtgccacctg

pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077) Sequences (1)

Full Sequences from Addgene (1)

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