pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296) Sequences (1)

Full Sequences from Addgene (1)

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  > Addgene NGS Result ggtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatata tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggag tatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacg tcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggca gtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctcccc atctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatggggg cggggggggggggggsgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagag gtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcg gccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctcc gccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacg gcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtga aagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtg tgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggc tttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctg cgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtc gggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctcc gtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggc ggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcga ggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtccc aaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtg cggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccct ctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggct tctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagct cctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattctgcagtcgacggtaccgc gggcccgggatccaccggtcgccaccatgggcagctcagagactggcccagtggctgtggaccccacatt gagacggcggatcgagccccatgagtttgaggtattcttcgatccgagagagctccgcaaggagacctgc ctgctttacgaaattaattgggggggccggcactccatttggcgacatacatcacagaacactaacaagc acgtcgaagtcaacttcatcgagaagttcacgacagaaagatatttctgtccgaacacaaggtgcagcat tacctggtttctcagctggagcccatgcggcgaatgtagtagggccatcactgaattcctgtcaaggtat ccccacgtcactctgtttatttacatcgcaaggctgtaccaccacgctgacccccgcaatcgacaaggcc tgcgggatttgatctcttcaggtgtgactatccaaattatgactgagcaggagtcaggatactgctggag aaactttgtgaattatagcccgagtaatgaagcccactggcctaggtatccccatctgtgggtacgactg tacgttcttgaactgtactgcatcatactgggcctgcctccttgtctcaacattctgagaaggaagcagc cacagctgacattctttaccatcgctcttcagtcttgtcattaccagcgactgcccccacacattctctg ggccaccgggttgaaatctggtggttcttctggtggttctagcggcagcgagactcccgggacctcagag tccgccacacccgaaagttccggagggagtagcggcgggtctacacagttcgagggctttaccaacctgt atcaggtgagcaagacactgcggtttgagctgatcccacagggcaagaccctgaagcacatccaggagca gggcttcatcgaggaggacaaggcccgcaatgatcactacaaggagctgaagcccatcatcgatcggatc tacaagacctatgccgaccagtgcctgcagctggtgcagctggattgggagaacctgagcgccgccatcg actcctatagaaaggagaaaaccgaggagacaaggaacgccctgatcgaggagcaggccacatatcgcaa tgccatccacgactacttcatcggccggacagacaacctgaccgatgccatcaataagagacacgccgag atctacaagggcctgttcaaggccgagctgtttaatggcaaggtgctgaagcagctgggcaccgtgacca caaccgagcacgagaacgccctgctgcggagcttcgacaagtttacaacctacttctccggcttttatag aaacaggaagaacgtgttcagcgccgaggatatcagcacagccatcccacaccgcatcgtgcaggacaac ttccccaagtttaaggagaattgtcacatcttcacacgcctgatcaccgccgtgcccagcctgcgggagc actttgagaacgtgaagaaggccatcggcatcttcgtgagcacctccatcgaggaggtgttttccttccc tttttataaccagctgctgacacagacccagatcgacctgtataaccagctgctgggaggaatctctcgg gaggcaggcaccgagaagatcaagggcctgaacgaggtgctgaatctggccatccagaagaatgatgaga cagcccacatcatcgcctccctgccacacagattcatccccctgtttaagcagatcctgtccgataggaa caccctgtctttcatcctggaggagtttaagagcgacgaggaagtgatccagtccttctgcaagtacaag acactgctgagaaacgagaacgtgctggagacagccgaggccctgtttaacgagctgaacagcatcgacc tgacacacatcttcatcagccacaagaagctggagacaatcagcagcgccctgtgcgaccactgggatac actgaggaatgccctgtatgagcggagaatctccgagctgacaggcaagatcaccaagtctgccaaggag aaggtgcagcgcagcctgaagcacgaggatatcaacctgcaggagatcatctctgccgcaggcaaggagc tgagcgaggccttcaagcagaaaaccagcgagatcctgtcccacgcacacgccgccctggatcagccact gcctacaaccctgaagaagcaggaggagaaggagatcctgaagtctcagctggacagcctgctgggcctg taccacctgctggactggtttgccgtggatgagtccaacgaggtggaccccgagttctctgcccggctga ccggcatcaagctggagatggagccttctctgagcttctacaacaaggccagaaattatgccaccaagaa gccctactccgtggagaagttcaagctgaactttcagatgcctacactggccagaggctgggacgtgaat agagagaagaacaatggcgccatcctgtttgtgaagaacggcctgtactatctgggcatcatgccaaagc agaagggcaggtataaggccctgagcttcgagcccacagagaaaaccagcgagggctttgataagatgta ctatgactacttccctgatgccgccaagatgatcccaaagtgcagcacccagctgaaggccgtgacagcc cactttcagacccacacaacccccatcctgctgtccaacaatttcatcgagcctctggagatcacaaagg agatctacgacctgaacaatcctgagaaggagccaaagaagtttcagacagcctacgccaagaaaaccgg cgaccagaagggctacagagaggccctgtgcaagtggatcgacttcacaagggattttctgtccaagtat accaagacaacctctatcgatctgtctagcctgcggccatcctctcagtataaggacctgggcgagtact atgccgagctgaatcccctgctgtaccacatcagcttccagagaatcgccgagaaggagatcatggatgc cgtggagacaggcaagctgtacctgttccagatctataacaaggactttgccaagggccaccacggcaag cctaatctgcacacactgtattggaccggcctgttttctccagagaacctggccaagacaagcatcaagc tgaatggccaggccgagctgttctaccgccctaagtccaggatgaagaggatggcacaccggctgggaga gaagatgctgaacaagaagctgaaggatcagaaaaccccaatccccgacaccctgtaccaggagctgtac gactatgtgaatcacagactgtcccacgacctgtctgatgaggccagggccctgctgcccaacgtgatca ccaaggaggtgtctcacgagatcatcaaggataggcgctttaccagcgacaagttctttttccacgtgcc tatcacactgaactatcaggccgccaattccccatctaagttcaaccagagggtgaatgcctacctgaag gagcaccccgagacacctatcatcggcatcgcccggggcgagagaaacctgatctatatcacagtgatcg actccaccggcaagatcctggagcagcggagcctgaacaccatccagcagtttgattaccagaagaagct ggacaacagggagaaggagagggtggcagcaaggcaggcctggtctgtggtgggcacaatcaaggatctg aagcagggctatctgagccaggtcatccacgagatcgtggacctgatgatccactaccaggccgtggtgg tgctggagaacctgaatttcggctttaagagcaagaggaccggcatcgccgagaaggccgtgtaccagca gttcgagaagatgctgatcgataagctgaattgcctggtgctgaaggactatccagcagagaaagtggga ggcgtgctgaacccataccagctgacagaccagttcacctcctttgccaagatgggcacccagtctggct tcctgttttacgtgcctgccccatatacatctaagatcgatcccctgaccggcttcgtggaccccttcgt gtggaaaaccatcaagaatcacgagagccgcaagcacttcctggagggcttcgactttctgcactacgac gtgaaaaccggcgacttcatcctgcactttaagatgaacagaaatctgtccttccagaggggcctgcccg gctttatgcctgcatgggatatcgtgttcgagaagaacgagacacagtttgacgccaagggcaccccttt catcgccggcaagagaatcgtgccagtgatcgagaatcacagattcaccggcagataccgggacctgtat cctgccaacgagctgatcgccctgctggaggagaagggcatcgtgttcagggatggctccaacatcctgc caaagctgctggagaatgacgattctcacgccatcgacacgatggtggccctgatccgcagcgtgctgca gatgcggaactccaatgccgccacaggcgaggactatatcaacagccccgtgcgcgatctgaatggcgtg tgcttcgactcccggtttcagaacccagagtggccaatggacgccgatgccaatggcgcctaccacatcg ccctgaagggccagctgctgctgaatcacctgaaggagagcaaggatctgaagctgcagaacggcatctc caatcaggactggctggcctacatccaggagctgcgcaactctggtggttctggaggatctggtggttct actaatctgtcagatattattgaaaaggagaccggtaagcaactggttatccaggaatccatcctcatgc tcccagaggaggtggaagaagtcattgggaacaagccggaaagcgatatactcgtgcacaccgcctacga cgagagcaccgacgagaatgtcatgcttctgactagcgacgcccctgaatacaagccttgggctctggtc atacaggatagcaacggtgagaacaagattaagatgctctctggtggttctcccaagaagaagaggaaag tctaaagcggccgcactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccct ggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgag catctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctct cactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggc aacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacag ccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgtt ttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcct ctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgcagcccaagctt ggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacga gccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgct cactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagcaaccata gtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggct gactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgagg aggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgcagcttataatggttacaaat aaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaa actcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggagaggcg gtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcg agcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggc tccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactata aagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgga tacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagtt cggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctt atccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggt aacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct acactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtag ctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgc agaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaact cacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatg aagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgag gcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataacta cgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctcc agatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcc tccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacg ttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttc ccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccg atcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctctta ctgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtg tatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttta aaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcca gttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtg agcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcata ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaat gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctg