pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2) Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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> Addgene NGS Result gtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatat ggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagt atttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgt caatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcag tacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctcccca tctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggc ggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagagg tgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcgg ccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccg ccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacgg cccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaa agccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgt gcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggct ttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgc gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcg ggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccg tacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcg gggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgag gcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtccca aatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgc ggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctc tccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggctt ctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctc ctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattctgcagtcgacggtaccgcg ggcccgggatccaccggtcgccaccatgggcccaaagaaaaagaggaaagtcggcagtggacctaaaaag aaacgaaaggttgggtcaggtagctcagagactggcccagtggctgtggaccccacattgagacggcgga tcgagccccatgagtttgaggtattcttcgatccgagagagctccgcaaggagacctgcctgctttacga aattaattgggggggccggcactccatttggcgacatacatcacagaacactaacaagcacgtcgaagtc aacttcatcgagaagttcacgacagaaagatatttctgtccgaacacaaggtgcagcattacctggtttc tcagctggagcccatgcggcgaatgtagtagggccatcactgaattcctgtcaaggtatccccacgtcac tctgtttatttacatcgcaaggctgtaccaccacgctgacccccgcaatcgacaaggcctgcgggatttg atctcttcaggtgtgactatccaaattatgactgagcaggagtcaggatactgctggagaaactttgtga attatagcccgagtaatgaagcccactggcctaggtatccccatctgtgggtacgactgtacgttcttga actgtactgcatcatactgggcctgcctccttgtctcaacattctgagaaggaagcagccacagctgaca ttctttaccatcgctcttcagtcttgtcattaccagcgactgcccccacacattctctgggccaccgggt tgaaatctggtggttcttctggtggttctagcggcagcgagactcccgggacctcagagtccgccacacc cgaaagttccggagggagtagcggcgggtctacacagttcgagggctttaccaacctgtatcaggtgagc aagacactgcggtttgagctgatcccacagggcaagaccctgaagcacatccaggagcagggcttcatcg aggaggacaaggcccgcaatgatcactacaaggagctgaagcccatcatcgatcggatctacaagaccta tgccgaccagtgcctgcagctggtgcagctggattgggagaacctgagcgccgccatcgactcctataga aaggagaaaaccgaggagacaaggaacgccctgatcgaggagcaggccacatatcgcaatgccatccacg actacttcatcggccggacagacaacctgaccgatgccatcaataagagacacgccgagatctacaaggg cctgttcaaggccgagctgtttaatggcaaggtgctgaagcagctgggcaccgtgaccacaaccgagcac gagaacgccctgctgcggagcttcgacaagtttacaacctacttctccggcttttatagaaacaggaaga acgtgttcagcgccgaggatatcagcacagccatcccacaccgcatcgtgcaggacaacttccccaagtt taaggagaattgtcacatcttcacacgcctgatcaccgccgtgcccagcctgcgggagcactttgagaac gtgaagaaggccatcggcatcttcgtgagcacctccatcgaggaggtgttttccttccctttttataacc agctgctgacacagacccagatcgacctgtataaccagctgctgggaggaatctctcgggaggcaggcac cgagaagatcaagggcctgaacgaggtgctgaatctggccatccagaagaatgatgagacagcccacatc atcgcctccctgccacacagattcatccccctgtttaagcagatcctgtccgataggaacaccctgtctt tcatcctggaggagtttaagagcgacgaggaagtgatccagtccttctgcaagtacaagacactgctgag aaacgagaacgtgctggagacagccgaggccctgtttaacgagctgaacagcatcgacctgacacacatc ttcatcagccacaagaagctggagacaatcagcagcgccctgtgcgaccactgggatacactgaggaatg ccctgtatgagcggagaatctccgagctgacaggcaagatcaccaagtctgccaaggagaaggtgcagcg cagcctgaagcacgaggatatcaacctgcaggagatcatctctgccgcaggcaaggagctgagcgaggcc ttcaagcagaaaaccagcgagatcctgtcccacgcacacgccgccctggatcagccactgcctacaaccc tgaagaagcaggaggagaaggagatcctgaagtctcagctggacagcctgctgggcctgtaccacctgct ggactggtttgccgtggatgagtccaacgaggtggaccccgagttctctgcccggctgaccggcatcaag ctggagatggagccttctctgagcttctacaacaaggccagaaattatgccaccaagaagccctactccg tggagaagttcaagctgaactttcagatgcctacactggccagaggctgggacgtgaatagagagaagaa caatggcgccatcctgtttgtgaagaacggcctgtactatctgggcatcatgccaaagcagaagggcagg tataaggccctgagcttcgagcccacagagaaaaccagcgagggctttgataagatgtactatgactact tccctgatgccgccaagatgatcccaaagtgcagcacccagctgaaggccgtgacagcccactttcagac ccacacaacccccatcctgctgtccaacaatttcatcgagcctctggagatcacaaaggagatctacgac ctgaacaatcctgagaaggagccaaagaagtttcagacagcctacgccaagaaaaccggcgaccagaagg gctacagagaggccctgtgcaagtggatcgacttcacaagggattttctgtccaagtataccaagacaac ctctatcgatctgtctagcctgcggccatcctctcagtataaggacctgggcgagtactatgccgagctg aatcccctgctgtaccacatcagcttccagagaatcgccgagaaggagatcatggatgccgtggagacag gcaagctgtacctgttccagatctataacaaggactttgccaagggccaccacggcaagcctaatctgca cacactgtattggaccggcctgttttctccagagaacctggccaagacaagcatcaagctgaatggccag gccgagctgttctaccgccctaagtccaggatgaagaggatggcacaccggctgggagagaagatgctga acaagaagctgaaggatcagaaaaccccaatccccgacaccctgtaccaggagctgtacgactatgtgaa tcacagactgtcccacgacctgtctgatgaggccagggccctgctgcccaacgtgatcaccaaggaggtg tctcacgagatcatcaaggataggcgctttaccagcgacaagttctttttccacgtgcctatcacactga actatcaggccgccaattccccatctaagttcaaccagagggtgaatgcctacctgaaggagcaccccga gacacctatcatcggcatcgcccggggcgagagaaacctgatctatatcacagtgatcgactccaccggc aagatcctggagcagcggagcctgaacaccatccagcagtttgattaccagaagaagctggacaacaggg agaaggagagggtggcagcaaggcaggcctggtctgtggtgggcacaatcaaggatctgaagcagggcta tctgagccaggtcatccacgagatcgtggacctgatgatccactaccaggccgtggtggtgctggagaac ctgaatttcggctttaagagcaagaggaccggcatcgccgagaaggccgtgtaccagcagttcgagaaga tgctgatcgataagctgaattgcctggtgctgaaggactatccagcagagaaagtgggaggcgtgctgaa cccataccagctgacagaccagttcacctcctttgccaagatgggcacccagtctggcttcctgttttac gtgcctgccccatatacatctaagatcgatcccctgaccggcttcgtggaccccttcgtgtggaaaacca tcaagaatcacgagagccgcaagcacttcctggagggcttcgactttctgcactacgacgtgaaaaccgg cgacttcatcctgcactttaagatgaacagaaatctgtccttccagaggggcctgcccggctttatgcct gcatgggatatcgtgttcgagaagaacgagacacagtttgacgccaagggcacccctttcatcgccggca agagaatcgtgccagtgatcgagaatcacagattcaccggcagataccgggacctgtatcctgccaacga gctgatcgccctgctggaggagaagggcatcgtgttcagggatggctccaacatcctgccaaagctgctg gagaatgacgattctcacgccatcgacacgatggtggccctgatccgcagcgtgctgcagatgcggaact ccaatgccgccacaggcgaggactatatcaacagccccgtgcgcgatctgaatggcgtgtgcttcgactc ccggtttcagaacccagagtggccaatggacgccgatgccaatggcgcctaccacatcgccctgaagggc cagctgctgctgaatcacctgaaggagagcaaggatctgaagctgcagaacggcatctccaatcaggact ggctggcctacatccaggagctgcgcaacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaa gaaaaagggatcctctggtggttctggaggatctggtggttctactaatctgtcagatattattgaaaag gagaccggtaagcaactggttatccaggaatccatcctcatgctcccagaggaggtggaagaagtcattg ggaacaagccggaaagcgatatactcgtgcacaccgcctacgacgagagcaccgacgagaatgtcatgct tctgactagcgacgcccctgaatacaagccttgggctctggtcatacaggatagcaacggtgagaacaag attaagatgctctctggtggttctcccaagaagaagaggaaagtctaaagcggccgcactcctcaggtgc aggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttt tccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaa tttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaa atcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccat gaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccat agaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccc taaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgt ccctcttctcttatggagatccctcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttcc tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctgg ggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacc tgtcgtgccagcggatccgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccg cccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagagg ccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttg caaaaagctaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaa ataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctg gatccgctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgctt cctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcc aggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaag ctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgg gctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaa cccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgta ggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatct gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgc tggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagat tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatata tgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctattt cgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcc ccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagc cggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtgg tgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatc ccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgca gtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgctttt ctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgccc ggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttct tcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcaccca actgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgc aaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagc atttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagggg ttccgcgcacatttccccgaaaagtgccacctgg

pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2) Sequences (1)

Full Sequences from Addgene (1)

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