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Addgene

pFastBac-His6-Mps1-KD
(Plasmid #114064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mps1-KD
  • Species
    H. sapiens (human)
  • Mutation
    Mps1 KD (D664A)
  • Entrez Gene
    TTK (a.k.a. CT96, ESK, MPH1, MPS1, MPS1L1, PYT)
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-His6-Mps1-KD was a gift from Geert Kops (Addgene plasmid # 114064 ; http://n2t.net/addgene:114064 ; RRID:Addgene_114064)

  • For your References section:

    Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Jelluma N, Brenkman AB, van den Broek NJ, Cruijsen CW, van Osch MH, Lens SM, Medema RH, Kops GJ. Cell. 2008 Jan 25;132(2):233-46. doi: 10.1016/j.cell.2007.11.046. 10.1016/j.cell.2007.11.046 PubMed 18243099
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