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pLAP-MIS12-KARD-3A
(Plasmid #114059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIC58
  • Backbone manufacturer
    Cheeseman IM and Desai A, Science's STKE 2005;2005:l1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIS12-KARD-3A
  • Species
    H. sapiens (human)
  • Mutation
    BUBR1-S670A, S676A and T680A
  • Entrez Gene
    BUB1B (a.k.a. BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1)
  • Tag / Fusion Protein
    • YFP-TEV-S- Human LAP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

based on pLAP-MIS12-KARD: point muations are not included in Sequence:partial 2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLAP-MIS12-KARD-3A was a gift from Geert Kops (Addgene plasmid # 114059 ; http://n2t.net/addgene:114059 ; RRID:Addgene_114059)

  • For your References section:

    Integration of kinase and phosphatase activities by BUBR1 ensures formation of stable kinetochore-microtubule attachments. Suijkerbuijk SJ, Vleugel M, Teixeira A, Kops GJ. Dev Cell. 2012 Oct 16;23(4):745-55. doi: 10.1016/j.devcel.2012.09.005. 10.1016/j.devcel.2012.09.005 PubMed 23079597
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