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pLacI-GFP-HEC1-d207
(Plasmid #114050)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114050 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLacI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-HEC1-d207
  • Species
    H. sapiens (human)
  • Mutation
    HEC1 delta 207
  • Entrez Gene
    NDC80 (a.k.a. HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLacI-GFP-HEC1-d207 was a gift from Geert Kops (Addgene plasmid # 114050 ; http://n2t.net/addgene:114050 ; RRID:Addgene_114050)

  • For your References section:

    A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B. Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ. J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. Epub 2013 Apr 8. 10.1083/jcb.201210033 PubMed 23569217
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