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Addgene

pCDNA5-LAP-MPS1-dTPR
(Plasmid #114048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114048 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MPS1-dTPR
  • Species
    H. sapiens (human)
  • Mutation
    delta TPR (deletion of TPR domain 63-191)
  • Entrez Gene
    TTK (a.k.a. CT96, ESK, MPH1, MPS1, MPS1L1, PYT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5-LAP-MPS1-dTPR was a gift from Geert Kops (Addgene plasmid # 114048 ; http://n2t.net/addgene:114048 ; RRID:Addgene_114048)

  • For your References section:

    A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B. Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ. J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. Epub 2013 Apr 8. 10.1083/jcb.201210033 PubMed 23569217
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