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MIS12-FRB-FLAG
(Plasmid #114040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.0
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIS12
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIS12 (a.k.a. 2510025F08Rik, KNTC2AP, MTW1, hMis12)
  • Promoter CMV
  • Tag / Fusion Protein
    • FRB-FLAG-tagRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T2098L mutant of FKBP-rapamycin binding domain of human FRAP. Puromycin is expressed via IRES sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIS12-FRB-FLAG was a gift from Geert Kops (Addgene plasmid # 114040 ; http://n2t.net/addgene:114040 ; RRID:Addgene_114040)

  • For your References section:

    Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Kuijt TE, Omerzu M, Saurin AT, Kops GJ. Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4. 10.1007/s00412-014-0458-9 PubMed 24695965
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