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Addgene

pcDNA5-eYFP-FKBP-MAD1-AA
(Plasmid #114038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human MAD1
  • Species
    H. sapiens (human)
  • Mutation
    MAD2 binding deficient MAD1 K541A/L543A
  • Entrez Gene
    MAD1L1 (a.k.a. MAD1, MVA7, PIG9, TP53I9, TXBP181)
  • Promoter CMV
  • Tag / Fusion Protein
    • eYFP-FKBP12 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-eYFP-FKBP-MAD1-AA was a gift from Geert Kops (Addgene plasmid # 114038 ; http://n2t.net/addgene:114038 ; RRID:Addgene_114038)

  • For your References section:

    Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Kuijt TE, Omerzu M, Saurin AT, Kops GJ. Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4. 10.1007/s00412-014-0458-9 PubMed 24695965
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