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pcDNA5-puro-eGFP-HEC1-M2
(Plasmid #114035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP tagged - HEC1 -M2
  • Alt name
    NDC80
  • Species
    H. sapiens (human)
  • Mutation
    M2 (K81E, N87E, K89E)
  • Entrez Gene
    NDC80 (a.k.a. HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80)
  • Promoter CMV TetO

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-puro-eGFP-HEC1-M2 was a gift from Geert Kops (Addgene plasmid # 114035 ; http://n2t.net/addgene:114035 ; RRID:Addgene_114035)

  • For your References section:

    CELL DIVISION CYCLE. Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling. Hiruma Y, Sacristan C, Pachis ST, Adamopoulos A, Kuijt T, Ubbink M, von Castelmur E, Perrakis A, Kops GJ. Science. 2015 Jun 12;348(6240):1264-7. doi: 10.1126/science.aaa4055. Epub 2015 Jun 11. 10.1126/science.aaa4055 PubMed 26068855
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