pcDNA5-hygro-eGFP-HEC1
(Plasmid
#114027)
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PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT/TO - hygromycin
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP-HEC1 - Wildtype (NDC80)
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SpeciesH. sapiens (human)
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Entrez GeneNDC80 (a.k.a. HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-hygro-eGFP-HEC1 was a gift from Geert Kops (Addgene plasmid # 114027 ; http://n2t.net/addgene:114027 ; RRID:Addgene_114027) -
For your References section:
Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Etemad B, Kuijt TE, Kops GJ. Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987. 10.1038/ncomms9987 PubMed 26621779