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Addgene

pcDNA5-puro-eGFP-HEC1-9A
(Plasmid #114023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO - puromycin
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP-HEC1 - 9A (NDC80)
  • Species
    H. sapiens (human)
  • Mutation
    Hec1 - 9A
  • Entrez Gene
    NDC80 (a.k.a. HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-puro-eGFP-HEC1-9A was a gift from Geert Kops (Addgene plasmid # 114023 ; http://n2t.net/addgene:114023 ; RRID:Addgene_114023)

  • For your References section:

    Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Etemad B, Kuijt TE, Kops GJ. Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987. 10.1038/ncomms9987 PubMed 26621779
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