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Addgene

pLV-CMV-tagRFP-Mad2
(Plasmid #114021)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tagRFP-Mad2 (MAD2L1)
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAD2L1 (a.k.a. HSMAD2, MAD2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-CMV-tagRFP-Mad2 was a gift from Geert Kops (Addgene plasmid # 114021 ; http://n2t.net/addgene:114021 ; RRID:Addgene_114021)

  • For your References section:

    Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Etemad B, Kuijt TE, Kops GJ. Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987. 10.1038/ncomms9987 PubMed 26621779
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