CFP-PBD(H83,86L)
(Plasmid #11402)

• Depositing Lab: Joel Swanson
• Publication: Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Epub 2004 May 28.

Backbone

• Vector backbone: pECFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p21-activated kinase 1
• Alt name: PAK1
• Alt name: PBD(H83,86L)
• Species: H. sapiens (human)
• Insert Size (bp): 155
• Mutation: Obtained with Histidine residues 83 and 86 changed to Leucine.
• GenBank ID: NM_002576
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Tag / Fusion Protein:
  • CFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GGTGGGAGGTCTATATAAGCAGAGCTGGTT
• 3′ sequencing primer: CCCCGGTGAACAGCTCCTCGCCCTTGCTCA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid encoding a mutant, Cdc42/Rac-binding deficient p21-binding domain (PBD(LL)) from human PAK1 was a gift from Gary Bokoch.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes PBD(LL) with a N-terminal CFP marker. CFP is the monomeric (A207K) version of ECFP.