sgTrack-Gateway
(Plasmid
#114011)
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Purpose(Empty Backbone) 3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114011 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiCRISPRv2
- Backbone size (bp) 12489
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Modifications to backboneReplaced Cas9 with Gateway cassette
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgTrack-Gateway was a gift from Charles Rudin (Addgene plasmid # 114011 ; http://n2t.net/addgene:114011 ; RRID:Addgene_114011) -
For your References section:
Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics. Hulton CH, Costa EA, Shah NS, Quintanal-Villalonga A, Heller G, de Stanchina E, Rudin CM, Poirier JT. Nat Cancer. 2020 Mar;1(3):359-369. doi: 10.1038/s43018-020-0040-8. Epub 2020 Mar 9. 10.1038/s43018-020-0040-8 PubMed 33345196
Map uploaded by the depositor.
