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Addgene

pSpCTRE-CD4
(Plasmid #114010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-TetOne-Puro
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    CD4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Alt name
    Cas9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4104

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCTRE-CD4 was a gift from Charles Rudin (Addgene plasmid # 114010 ; http://n2t.net/addgene:114010 ; RRID:Addgene_114010)

  • For your References section:

    Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics. Hulton CH, Costa EA, Shah NS, Quintanal-Villalonga A, Heller G, de Stanchina E, Rudin CM, Poirier JT. Nat Cancer. 2020 Mar;1(3):359-369. doi: 10.1038/s43018-020-0040-8. Epub 2020 Mar 9. 10.1038/s43018-020-0040-8 PubMed 33345196
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