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pHAGE-EF1a-MICB*005-IRES-ZsGreen
(Plasmid #114008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-CMV-fullEF1a-IRES-ZsGreen
  • Backbone manufacturer
    Harvard Medical School
  • Backbone size w/o insert (bp) 8346
  • Total vector size (bp) 9372
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    ZsGreen

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MICB*005
  • Alt name
    MICB
  • Alt name
    C1601
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1026
  • Entrez Gene
    MICB (a.k.a. PERB11.2)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer AAAAAAGCGG CCGCATGGGGCTGG GCCGGGTCC
  • 3′ sequencing primer AAAAAAGGAT CCCTAGGCGCCCTCAGTGGAACCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-EF1a-MICB*005-IRES-ZsGreen was a gift from Kai Wucherpfennig (Addgene plasmid # 114008 ; http://n2t.net/addgene:114008 ; RRID:Addgene_114008)

  • For your References section:

    Antibody-mediated inhibition of MICA and MICB shedding promotes NK cell-driven tumor immunity. Ferrari de Andrade L, Tay RE, Pan D, Luoma AM, Ito Y, Badrinath S, Tsoucas D, Franz B, May KF Jr, Harvey CJ, Kobold S, Pyrdol JW, Yoon C, Yuan GC, Hodi FS, Dranoff G, Wucherpfennig KW. Science. 2018 Mar 30;359(6383):1537-1542. doi: 10.1126/science.aao0505. 10.1126/science.aao0505 PubMed 29599246
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