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pHAGE-EF1a MICA*009-IRES-ZsGreen
(Plasmid #114007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-CMV-fullEF1a-IRES-ZsGreen
  • Backbone manufacturer
    Harvard Medical School
  • Backbone size w/o insert (bp) 8346
  • Total vector size (bp) 9504
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    ZsGreen

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MICA*009
  • Alt name
    MICA
  • Alt name
    C1444
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1158
  • Entrez Gene
    MICA (a.k.a. MIC-A, PERB11.1)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer AAAAAAGCGGCCGCATGGGGCTGGGCCCGGTCTTC
  • 3′ sequencing primer AAAAAAGGATCCCTAGGCGCCCTCAGTGGAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-EF1a MICA*009-IRES-ZsGreen was a gift from Kai Wucherpfennig (Addgene plasmid # 114007 ; http://n2t.net/addgene:114007 ; RRID:Addgene_114007)

  • For your References section:

    Antibody-mediated inhibition of MICA and MICB shedding promotes NK cell-driven tumor immunity. Ferrari de Andrade L, Tay RE, Pan D, Luoma AM, Ito Y, Badrinath S, Tsoucas D, Franz B, May KF Jr, Harvey CJ, Kobold S, Pyrdol JW, Yoon C, Yuan GC, Hodi FS, Dranoff G, Wucherpfennig KW. Science. 2018 Mar 30;359(6383):1537-1542. doi: 10.1126/science.aao0505. 10.1126/science.aao0505 PubMed 29599246
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