pOGG216
(Plasmid
#114002)
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Purpose(Empty Backbone) Level 2 golden gate cloning vector for medium copy number free-living expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepL2V-L2-tet-pBBR-ELT3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationDomesticated for Golden Gate cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pL2V-L2-tet-pBBR-ELT3. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOGG216 was a gift from Philip Poole (Addgene plasmid # 114002 ; http://n2t.net/addgene:114002 ; RRID:Addgene_114002) -
For your References section:
A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors. Geddes BA, Mendoza-Suarez MA, Poole PS. Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. eCollection 2018. 10.3389/fmicb.2018.03345 PubMed 30692983
