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Addgene

pOGG082
(Plasmid #114000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pL0M-PU-pNifH
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NifH promoter
  • Species
    Synthetic
  • Mutation
    Domesticated for Golden Gate cloning

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pL0M-PU-pNifH. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOGG082 was a gift from Philip Poole (Addgene plasmid # 114000 ; http://n2t.net/addgene:114000 ; RRID:Addgene_114000)

  • For your References section:

    A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors. Geddes BA, Mendoza-Suarez MA, Poole PS. Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. eCollection 2018. 10.3389/fmicb.2018.03345 PubMed 30692983
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