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pCEP4-HLA-c-myc-optiT1R3 ECD
(Plasmid #113960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 10393
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T1R3
  • Alt name
    hT1R3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1755
  • Mutation
    residues 21-563
  • GenBank ID
    NM_152228.2
  • Entrez Gene
    TAS1R3 (a.k.a. T1R3)
  • Promoter cmv
  • Tags / Fusion Proteins
    • Signal/leader sequence from HLA class I histocompatibility antigen A-2 alpha chain (HLA) (N terminal on insert)
    • c-myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-HLA-c-myc-optiT1R3 ECD was a gift from Erik Procko (Addgene plasmid # 113960 ; http://n2t.net/addgene:113960 ; RRID:Addgene_113960)

  • For your References section:

    Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits. Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E. J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173. 10.1074/jbc.RA118.006173 PubMed 30723160
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