pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G
(Plasmid #113953)

• Purpose: mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
• Depositing Lab: Erik Procko
• Publication: Park et al J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 10393
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T1R2
• Alt name: natT1R2
• Alt name: hT1R2
• Species: H. sapiens (human)
• Insert Size (bp): 2553
• Mutation: delete N-terminal 21 residues, I132S, S212N, and D231G substitution
• GenBank ID: NM_152232.2
• Entrez Gene: TAS1R2 (a.k.a. GPR71, T1R2, TR2)
• Promoter: cmv
• Tags / Fusion Proteins:
  • Signal/leader sequence from influenza hemagglutinin (HA) (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry