pcDNA3.1(+)-HLA-Flag-natT1R3
(Plasmid #113950)

• Purpose: mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
• Depositing Lab: Erik Procko
• Publication: Park et al J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5344
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T1R3
• Alt name: hT1R3
• Species: H. sapiens (human)
• Insert Size (bp): 2622
• Mutation: residues 21-852
• GenBank ID: NM_152228.2
• Entrez Gene: TAS1R3 (a.k.a. T1R3)
• Tags / Fusion Proteins:
  • Signal/leader sequence from HLA class I histocompatibility antigen A-2 alpha chain (HLA) (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: BGH_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry