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Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5344
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT1R2
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Alt namenatT1R2
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Alt namehT1R2
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SpeciesH. sapiens (human)
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Insert Size (bp)2553
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Mutationresidues 22-839
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GenBank IDNM_152232.2
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Entrez GeneTAS1R2 (a.k.a. GPR71, T1R2, TR2)
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Tags
/ Fusion Proteins
- Signal/leader sequence from influenza hemagglutinin (HA) (N terminal on insert)
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer BGH_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-HA-Flag-natT1R2 was a gift from Erik Procko (Addgene plasmid # 113944 ; http://n2t.net/addgene:113944 ; RRID:Addgene_113944) -
For your References section:
Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits. Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E. J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173. 10.1074/jbc.RA118.006173 PubMed 30723160