pBAD-R-GECO1
(Plasmid #113943)

• Purpose: Bacterial expression of red fluorescent Ca2+ sensor R-GECO1
• Depositing Labs: Robert Campbell, Thomas Hughes
• Publication: Molina et al Biophys J. 2019 May 21;116(10):1873-1886. doi: 10.1016/j.bpj.2019.04.007. Epub 2019 Apr 13.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: ThermoFisher Scientific
• Backbone size w/o insert (bp): 4067
• Total vector size (bp): 5318
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For inducing protein expression with arabinose, grow in DH10B cells.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: R-GECO1
• Species: Synthetic
• Insert Size (bp): 1251
• GenBank ID: JN258411
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBAD Forward
• 3′ sequencing primer: pBAD Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Robert Campbell, University of Alberta.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/10/04/435891 for bioRxiv preprint.