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Addgene

pBAD-CAR-GECO1
(Plasmid #113939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 4067
  • Total vector size (bp) 5318
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For inducing protein expression with arabinose, grow in DH10B cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAR-GECO1
  • Species
    Synthetic
  • Insert Size (bp)
    1251
  • GenBank ID
    KF186684
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Robert Campbell, University of Alberta.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/10/04/435891 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-CAR-GECO1 was a gift from Robert Campbell & Thomas Hughes (Addgene plasmid # 113939 ; http://n2t.net/addgene:113939 ; RRID:Addgene_113939)

  • For your References section:

    Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators. Molina RS, Qian Y, Wu J, Shen Y, Campbell RE, Drobizhev M, Hughes TE. Biophys J. 2019 May 21;116(10):1873-1886. doi: 10.1016/j.bpj.2019.04.007. Epub 2019 Apr 13. 10.1016/j.bpj.2019.04.007 PubMed 31054773
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