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Addgene

mCherry-OptoPB
(Plasmid #113935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6219
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OptoPB
  • Alt name
    mCh-LOV2-Rit-PB
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1263
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer mch-for: CCC CGT AAT GCA GAA GAA GA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-OptoPB was a gift from Yubin Zhou (Addgene plasmid # 113935 ; http://n2t.net/addgene:113935 ; RRID:Addgene_113935)

  • For your References section:

    Optical control of membrane tethering and interorganellar communication at nanoscales. He L, Jing J, Zhu L, Tan P, Ma G, Zhang Q, Nguyen NT, Wang J, Zhou Y, Huang Y. Chem Sci. 2017 Aug 1;8(8):5275-5281. doi: 10.1039/c7sc01115f. Epub 2017 May 31. c7sc01115f [pii] PubMed 28959426
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