GFP-LiMETER-v2
(Plasmid #113934)

• Purpose: Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
• Depositing Lab: Yubin Zhou
• Publication: He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi: 10.1039/c7sc01115f. Epub 2017 May 31.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LOV2 and Stim1 polybasic domain (PB)
• Alt name: LiMETER-V2
• Species: Synthetic
• Insert Size (bp): 1500
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMVf: CGC AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: BGHrev: TAG AAG GCA CAG TCG AGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry