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Addgene

GFP-LiMETER-v1
(Plasmid #113933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LOV2 and Rit polybasic domain (PB)
  • Alt name
    LiMETER-V1
  • Species
    Synthetic
  • Insert Size (bp)
    1500
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMVf: CGC AAA TGG GCG GTA GGC GTG
  • 3′ sequencing primer BGHrev: TAG AAG GCA CAG TCG AGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-LiMETER-v1 was a gift from Yubin Zhou (Addgene plasmid # 113933 ; http://n2t.net/addgene:113933 ; RRID:Addgene_113933)

  • For your References section:

    Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca(2+) influx. Jing J, He L, Sun A, Quintana A, Ding Y, Ma G, Tan P, Liang X, Zheng X, Chen L, Shi X, Zhang SL, Zhong L, Huang Y, Dong MQ, Walker CL, Hogan PG, Wang Y, Zhou Y. Nat Cell Biol. 2015 Oct;17(10):1339-47. doi: 10.1038/ncb3234. Epub 2015 Aug 31. 10.1038/ncb3234 PubMed 26322679
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